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  • Microbial Community Composition in lakes - Ecological characteristics of the sample at North Temperate Lakes LTER 2002 - 2007
  • Kent, Angela; NTL LTER
    McMahon, Katherine; University of Wisconsin
    Newton, Ryan; NTL LTER
    Yannarell, Anthony; NTL LTER
  • 2008
  • Kent, A., K. McMahon, R. Newton, and A. Yannarell. 2013. Microbial Community Composition in lakes - Ecological characteristics of the sample at North Temperate Lakes LTER 2002 - 2007 ver 2. Environmental Data Initiative. https://doi.org/10.6073/pasta/b0a645e44f9407c8b3e7c087bf3dbee9 (Accessed 2025-07-25).
  • Microbial community composition is inferred by a combination of automated ribosomal intergenic spacer analysis (ARISA) and PCR-generated clone library analysis. Clone libraries include both the 16S rRNA gene and the 16S-23S ribosomal intergenic spacer fragment. Phylogenetic assignments for individual ARISA fragments are obtained by comparing the ARISA fragment length from each clone to all of the profiles stored in our database. We have analyzed over 3900 clones obtained from 41 lakes that represent the range of trophic types found in temperate landscapes. Querying by ecological characteristics of the sample allows the user to retrieve sample IDs, sample dates, lake information (region, type, size, depth) and physical/chemical data (water temperature, clarity, pH, DOC, SUVA, TN, TP, nitrates/nitrites). The data can be filtered by lake name, sample date, lake information (region, type, size, depth), and physical/chemical data (water temperature, clarity, pH, DOC, SUVA, TN, TP, nitrates/nitrites). The output includes links to individual sample records, which contain links to the taxonomic composition of the sample inferred by dynamically matching clones to ARISA fragments in the individual sample

  • N: 46.203      S: 42.798      E: -88.93      W: -90.11
  • Data Policies Copyright Board of Regents, University of Wisconsin, Madison. This information is released to the public and may be used for academic, educational, or commercial purposes subject to the following restrictions: The Data User must realize that these data sets are being actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the NTL lead Principal Investigator, leadpi@lter.limnology.wisc.edu, to check on other uses of the data. Where appropriate, the Data User may be encouraged to consider collaboration and/or co-authorship with original investigators. The Data User must realize that the data may be misinterpreted if taken out of context. We request that you provide the NTL lead Principal Investigator, ATTN: Data Access, Center for Limnology, University of Wisconsin-Madison, 680 North Park St., Madison, WI 53706 with a copy of any manuscript using the data so that it may be reviewed and comments provided on the presentation of our data. The Data User must acknowledge use of the data by an appropriate citation (see Citation) of the NTL-LTER database. A generic citation for our databases is: name of data set, North Temperate Lakes Long Term Ecological Research program, NSF, contact person for data set, Center for Limnology, University of Wisconsin-Madison. The data set name and contact person for each data set can be found in the metadata header of the online data sets. The Data User must send two reprints of any publications resulting from use of the data to the address above. We would like to include such manuscripts in our LTER publications list. The Data User must not redistribute original data and documentation without permission from Emily Stanley, lead Principal Investigator, (ehstanley@wisc.edu). While substantial efforts are made to ensure the accuracy of data and documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is". The North Temperate Lakes LTER shall not be liable for damages resulting from any use or misinterpretation of data sets. Data users should be aware that we periodically update data sets. Our goal is to release all long term data associated with core research areas within 2 years of collection. These data and accompanying metadata will be available for download from the NTL-LTER web site. By using these data, the Data User agrees to abide by the terms of this agreement. Thank you for your cooperation.
  • https://doi.org/10.6073/pasta/b0a645e44f9407c8b3e7c087bf3dbee9
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