Size group (pico, nano, micro) and group total carbon estimates from cell counts via epifluorescent microscopy (EPI) of heterotrophic and autotrophic plankton from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2011 (ongoing).
California Current Ecosystem LTER
Landry, Michael; SIO
Landry M. 2017. Size group (pico, nano, micro) and group total carbon estimates from cell counts via epifluorescent microscopy (EPI) of heterotrophic and autotrophic plankton from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2011 (ongoing).. Environmental Data Initiative. http://dx.doi.org/10.6073/pasta/658bbc48584ef46af05aa745b2eb252d. Dataset accessed 3/24/2018.
Microbial community assemblages of the California Current Ecosystem (CCE) are assessed for biomass of heterotrophic (dinoflagellate and other eukaryotes) and autotrophic (dinoflagellate and other eukaryotes) plankton using high-throughput digital epifluorescence microscopy (EPI). Samples to estimate the nano- and microplankton (0.2-2.0-µm and 2.0-20-µm size, respectively) are collected at various depths via Niskin bottles, preserved, stained, and filtered onto a membrane filter and mounted on a glass microscope slide aboard the quarterly CalCOFI survey cruises (since 2004, ongoing). Slides are then frozen at -80°C for subsequent imaging and analysis in the laboratory onshore. Carbon biomass is computed from cell biovolumes.
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