In the Baltimore urban long-term ecological research (LTER) project, (Baltimore Ecosystem Study, BES) we use the watershed approach to evaluate integrated ecosystem function. The LTER research is centered on the Gwynns Falls watershed, a 17,150 ha catchment that traverses a gradient from the urban core of Baltimore, through older urban residential (1900 - 1950) and suburban (1950- 1980) zones, rapidly suburbanizing areas and a rural/suburban fringe. Stable isotopic analyses were carried out on stream samples collected bi-weekly from June 2005 through December 2005 as part of the routine Baltimore LTER sampling. Sites included POBR (forest), MCDN (agricultural), BARN (low-residential), GFGL (suburban), DRKR (urban), GFCP (urban), and RGHT (storm drain). Samples were also taken from a small tributary to the Gwynns Falls (GFGR), approximately 300 m above GFCP, that was highly contaminated with sewage. A major sewer leak to this stream was identified and repaired in April 2004. Stable isotopic analyses of soil water underneath fertilized lawns and atmospheric deposition was measured in long-term lawn study plots on the campus of the University of Maryland Baltimore County. Storm samples were also collected from 6 locations (DR1, DR3.1, DR3.2, DR4, DR5, DRKR gauge) within the Dead Run watershed over July 2005. At one site (DR3), two sets of samples were collected, one just above (DR3.1) and just below (DR3.2) an overflowing sewer. All stormflow samples were collected on the receding limb of the storm hydrograph, as the flashy nature of these urban streams makes sampling the rising limb difficult in terms of both timing and personal safety. Nitrate concentrations are a separate set of chemical analyses than the routine weekly analyses.